LOCUS       40924_30755        5680 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMG103 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Inui M, Roh JH, Zahn K, Yukawa H.
  TITLE     Sequence analysis of the cryptic plasmid pMG101 from 
            Rhodopseudomonas palustris and construction of stable cloning 
            vectors
  JOURNAL   Appl. Environ. Microbiol. 66 (1), 54-63 (2000)
  PUBMED    10618203
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Inui M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-1999) Masayuki Inui, Research Institute of 
            Innovative Technology for the Earth, Molecular Microbiology and 
            Genetics Laboratory; 9-2, Kizugawadai, Soraku, Kizu, Kyoto 619-0292,
            Japan (E-mail:inui@rite.or.jp, Tel:81-774-75-2308, 
            Fax:81-774-75-2321)
REFERENCE   3  (bases 1 to 5680)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5680)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2000"; volume: "66"; issue: "1"; pages:
            "54-63"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-AUG-1999) Masayuki Inui, Research Institute of Innovative 
            Technology for the Earth, Molecular Microbiology and Genetics 
            Laboratory"; volume: " 9-2, Kizugawadai, Soraku, Kizu, Kyoto 
            619-0292, Japan (E-mail:inui@rite.or.jp, Tel:81-774-75-2308, Fax"; 
            pages: "81-774-75-2321"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1..27
                     /mol_type="other DNA"
                     /label=derived from cloning vector pHSG298
                     /note="derived from cloning vector pHSG298"
                     /db_xref="taxon:32630"
                     /organism="synthetic construct"
     source          28..3080
                     /plasmid="pMG101"
                     /mol_type="other DNA"
                     /label=derived from 3.0-kb XhoI-SalI fragment of pMG101
                     /note="derived from 3.0-kb XhoI-SalI fragment of pMG101"
                     /db_xref="taxon:1076"
                     /organism="Rhodopseudomonas palustris"
     CDS             3331..4143
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4365..4381
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(4385..4441)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(4451..4467)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4475..4491)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4499..4529)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4544..4565)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5037..5625)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaggtctgcc tcgtgaagaa ggtgttgctc gagcgggtca tgaacaccct gccagatcga
       61 gaatcccgaa cttggcgcgc ggtattcttc aaggccctgc ctgaggtgat ggaggatttt
      121 gggggcgact ggctacaagc aactggcgaa cggcgaaatc cgttcgagcg ggtggaacag
      181 acgaagccaa taggaaggcc ataggcgcgt ttttatagac ttgaagcccg gaagtccgtc
      241 cacacccaaa aaatcgctgt gcgaggcgct gtggctgtcc tgtgttgcct tcgatgaaag
      301 gcgaaacttg ccctcatcat ggcagtcccc cctagtctgg tccttaaagc ggtcaagggc
      361 gatgccggag ggagcccttg agggcttgtg gggactaggc ttctttccct ttttcaatcg
      421 tctttttgaa gactgcttgt tctgaagatt ctgaaaagtg ttaggaagaa gtgttctgca
      481 atgttctgta gtgttctgtt ctatatagcg ccaccaagtc tgcggtagag cgctaccaag
      541 tctgcggaaa gccgccacca agtctgcggt agaagaagcg aaaacgccac caagtctgcg
      601 gtagagtgcc accaagtctg cggtggcggc ggaataagcc cttgaaatta ttgagtcccg
      661 ggaacagtaa ggcgccacca agtctgcgga gctaagtggt tgcgtttgcg gctacttttt
      721 gcgcacacgg atcgcaggta atgccagcac ggcggggtcg gctggttcga tggaaatgcc
      781 gtattgcgtg agcttgagag gcagctttgg cagcacttcc ttgatgtgtg tcagctcctc
      841 gatcagctgc gcccggaacg cgcgaggccg agtgaaacct tggccaaatt gttcgctgag
      901 agccttccaa ctcatggtca ccgggtggcg aatattgtga aggcgatagc ctagccagaa
      961 atacaaatcg agcttgcgag ctgagcctgc gaaggctcga accgcattag cattcaaggg
     1021 gagcgcacgc tgctgtaggc tctcaaagaa taccgggttg aacgtgacgg tactgggcca
     1081 aagcgagcgt tggtcaagat gcggcggtag ccacacctca acctcatcga acggattgac
     1141 ggttttggtt tttgccctca ccccgtccca cacactcacc cgcattgtgc aagccgccag
     1201 tgcgttaatt tgttctttga aggcggtgag cgggcctttg cgtcccccgc tgtccgaaaa
     1261 tcccatctcg cgcacaaagg cagtaaagct ctcggcgatt tcgatagtag gtgttttctg
     1321 ccggatggct tccgaacaaa ggtgcatcat caccagtcgg gcttttggcc cgaaaggcag
     1381 cggttgaagg attttcttgc cttctccgtc tcgcaggaat ccggcggaca aatccagcgc
     1441 catattgccc tgcttccgtt cgaactcgcg cacttcgaga ggttgacggt cgtaaggcat
     1501 accgcaaacg gcaagcacgc tgtgcaaatg ccgaacgtcg gcgggcgatg gcggtgagtt
     1561 ttcgataact tcgcggatga tggaacggcg gcggcgctcg cgtggcattg cgtcaagctc
     1621 agccttgcgc ttttcagcgg cggcctgttc ctcgtctcgc ttttgctggc gggtgatgag
     1681 cacgctgact tgggcatcaa acgcaaactt accccgcgct ttttcaagct cggcgcgcaa
     1741 atcttcatcc cggatcagcg attcgtcata cttgcccata ggcacagagt agctgatttg
     1801 ctgcggtctc cctaggcgcc tgcctgccta tagggcaatt acgtacagca ttatagcgtt
     1861 gtggcgttgt aacgctatag cattgcaatg ctaggacttg cgcttggccg tctcggagtg
     1921 gccgcgccta gcaatcacag catcgactcc ctcgacgatg aggtcatgca cctttttgcg
     1981 ctcgacaaag gcgatctctc gcagccgatc atgagccgct tggccaaata gatactggag
     2041 cgcactacag gagaggcttc agatggggga ggagcggctt ttggcgcgcg catcttcacc
     2101 accctggatt tcgacggagc cggagcgtca ggctccacct cgggggtgat ttctagccct
     2161 tcgatgatag gggggcgctt tttcggcggg ctcatgcgac ggctttcgtt ttgagtttgt
     2221 gctggatgga tctccagagt ccgcgggcct cctcggccgc cttgccgctg ggagcgtact
     2281 cggtgacccc ctgccctgcc gcgatggcat cctgaaagtc agccctagaa accatcaatg
     2341 gctctgcaag tgatcccagg gctgacaatc ccacggcggc ctcgctggcg cgcccgctgc
     2401 gggtgatcgg ggggcactgg ttgagaacaa acaggaatgg ccgctgcagc ttgagaagcg
     2461 cttggatggt cggccgggtt gcctgaatgt ctaggcgcgt cggcctagca ggcactaggg
     2521 agaggtcggc tgcctgcatg gcgagagagg tggcggtgct ggcgacaccc gggcaatcga
     2581 ggatggcgag ggtgtacccc ttccctgcca gcgccttcag gatttgaggg agctggggca
     2641 ccttgtcagg cggaagggca tcaacggcgg gcttctcccg ctgggcctga gcccgttgat
     2701 ctccccaagc tgcaagcgaa ccttgcgggt caaggtcgag ggcaatcacg gattcccctg
     2761 cctctgtggc cgcgacggca atggcagcag cgagggtagt ttttcccgcg ccgcccttct
     2821 gcgtgaccag agcaattgtc ttcatgcctg cactatagca ttaaggcact aaagcgtcaa
     2881 agcgccatag cggcatagcg gcatagcggc atagcgctaa aatgctatag cattattaaa
     2941 tacagcgcta cagcgctata atgctgcaac ggttaggacc gcaatttgcg ccccgggccg
     3001 gttgcgctat cgaccagctc aattaactgc tcgggctcgg acgcgaacca cgcgaagctg
     3061 ccccaagcca aggagtcgag ggagccacgg ttgatgagag ctttgttgta ggtggaccag
     3121 ttggtgattt tgaacttttg ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg
     3181 atctgatcct tcaactcagc aaaagttcga tttattcaac aaagccacgt tgtgtctcaa
     3241 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     3301 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc
     3361 tcgaagccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     3421 gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca
     3481 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     3541 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     3601 cctgatgatg catggttact caccactgcg atccccggga aaacagcatt ccaggtatta
     3661 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     3721 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     3781 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     3841 aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg
     3901 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     3961 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     4021 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     4081 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     4141 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     4201 gacggcggct ttgttgaata aatcgcattc gccattcagg ctgcgcaact gttgggaagg
     4261 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     4321 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     4381 tgccaagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg agctcgaatt
     4441 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     4501 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     4561 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     4621 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattggcgaa cttttgctga
     4681 gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg caaagcaaaa
     4741 gttcaaaatc agtaaccgtc agtgccgata agttcaaagt taaacctggt gttgatacca
     4801 acattgaaac gctgatcgaa aacgcgctga aaaacgctgc tgaatgtgcg agcttcttcc
     4861 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     4921 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     4981 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     5041 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     5101 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     5161 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     5221 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     5281 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     5341 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     5401 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     5461 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     5521 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     5581 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     5641 ttttctacgg ggtctgacgc tcagtggaac gatccgtcga
//
